Duff Post attended local schools before studying at Philadelphia Dental College to obtain a dentistry degree.
[1] Post established his dentistry practice in Tampa after graduating from college.
Post's first political office he would hold would be the President of the Hillsborough County Board of Health in 1873.
He would serve as Tampa's City Marshal from 1881 to 1883, and was Captain in the 4th Regiment of the Florida Militia.
[2] He opened an ice cream parlor and restaurant in the Masonic Lodge Building in downtown Tampa on March 31, 1877.