Dumfriesshire (Gaelic: Siorrachd Dhùn Phris) is a constituency of the Scottish Parliament (Holyrood) covering part of the council area of Dumfries and Galloway.

It is also one of nine constituencies in the South Scotland electoral region, which elects seven additional members, in addition to nine constituency MSPs, to produce a form of proportional representation for the region as a whole.

Created for the 2011 Scottish Parliament election, the constituency comprises areas that were previously part of the old Dumfries and Galloway and Upper Nithsdale constituencies, which were abolished and replaced by Dumfriesshire and Galloway and West Dumfries.

The other eight constituencies of the South Scotland region are: Ayr, Carrick, Cumnock and Doon Valley, Clydesdale, East Lothian, Ettrick, Roxburgh and Berwickshire, Galloway and West Dumfries, Kilmarnock and Irvine Valley and Midlothian South, Tweeddale and Lauderdale.

Dumfriesshire covers the eastern part of the council area.