He signed with the team soon after the draft,[1] and was assigned to the Rookie-level Gulf Coast League Blue Jays for the remainder of the season.

[2] Antolin remained with the Lugnuts in 2010, going 2–2 with a 2.93 ERA and 24 strikeouts in 272⁄3 innings pitched before undergoing Tommy John surgery.

Antolin made the leap to Dunedin full-time in 2012, making a career-high 48 relief appearances totaling 59 innings.

[2] He would be promoted to the Triple–A Buffalo Bisons in 2016, and opened the season with a 1–1 win–loss, 2.70 ERA, and 3 saves before being called up by the Blue Jays.

[10] On November 21, 2016, Antolin signed a minor league contract with the Washington Nationals that included an invitation to spring training.

[14] In the offseason, Antolin signed a minor-league contract with the Chicago White Sox,[15] but was released prior to the start of the season.