Holmes co-presented ITV's This Morning (2006–2021) with his then-wife Ruth Langsford on Fridays and during the school holidays.

Holmes was appointed Officer of the Order of the British Empire (OBE) for services to broadcasting in the 2018 New Year Honours.

He is an advocate of numerous charities and causes, including Dogs Trust, Variety GB and Northern Ireland Kidney Patients' Association.

[5] In addition to his news and current affairs role, Holmes has also hosted other Ulster Television productions, including Miss Northern Ireland gala specials in 1985 and 1986.

[5] At the corporation's Manchester studios, Holmes worked as a presenter on the daytime Open Air programme which was broadcast nationally on BBC1.

[7][8] He was also the presenter of ITV's coverage of the Phil Taylor vs. Raymond van Barneveld showdown at the Wembley Conference Centre in 1999.

[2] In April 2005, he left GMTV, stating the show had run out of real news and became celebrity obsessed shortly before his departure.

[12] After leaving GMTV in April 2005, Holmes joined Sky News six months later to present the new-look Sunrise programme.

[13] In 2014, Holmes and Ruth Langsford co-hosted a 10-part ITV daytime game show,[13] Gift Wrapped.

[15][16] In 2015, Holmes and Langsford presented a six-part factual series for Channel 5 entitled Eamonn & Ruth: How the Other Half Lives.

[20] In December 2021 it was announced that Holmes was joining GB News, where he began presenting its new breakfast show alongside Isabel Webster from 4 January 2022.

"[22] in February 2020, Holmes lost a First Tier Tribunal (FTT) appeal regarding his status as a freelancer under IR35 rules, with the judge determining his contract with ITV's This Morning programme amounted to employment.

Finally, on 19 December 2013, to celebrate the end of Millionaire on ITV, Holmes re-appeared on the show with Alex Ferguson.

[36] In November 2009, Jon Culshaw appeared as Holmes on The Impressions Show in a series of three comedy sketches in which he was portrayed as having an appetite so uncontrollable that he eats a sofa, flowers and even a guest (Frankie Dettori), using the catchphrase 'I was fierce hungry, so I was'.

Although Holmes had interviewed Culshaw and his co-star Debra Stephenson on This Morning to promote the show,[37] after the programme aired the presenter instructed his lawyers to send a letter of complaint to the BBC.

[38] The letter resulted in an apology from the BBC along with a withdrawal of any future comedy sketches featuring Holmes.

[39] Both the complaint itself and the outcome of Holmes's legal action drew generally unfavourable criticism from media observers and online commentators.

"[45] In 2020, during the COVID-19 pandemic, Holmes cause controversy by refusing to discount conspiracy theories linking 5G telecommunications masts to COVID-19.

[47] On 26 June 2010, Holmes married Ruth Langsford at Elvetham Hall, near Hartley Wintney, Hampshire.

[62] Later that year, Holmes discussed a series of health difficulties he had had, including spinal surgery and a double hip replacement, as well as severe back pain that had left him requiring a wheelchair.

[63][64] Holmes was appointed Officer of the Order of the British Empire (OBE) for services to broadcasting in the 2018 New Year Honours.