Roosting in caves and rock crevices during the day, it forages at night for insects near rivers and streams.

[2] Some authors previously considered it a subspecies of the long-fingered bat (Myotis capaccinii), though it is now widely recognized as a full species.

[1] It has three subspecies:[4] The eastern long-fingered bat is a social species, with males and females roosting together in colonies that can number several hundred individuals.

[1] It is insectivorous, catching its prey in flight (hawking) or plucking insects from the surface of bodies of water (gleaning).

[1] Within its range, the eastern long-fingered bat is strongly associated with riparian habitat, as it forages for food over rivers.