[3] The eastern sand darter feeds on larvae of black flies and other small insects in the water.
A. pellucida reproduces in the sandy shoals at the bottom of the lakes and rivers it inhabits during the spring and summer when the water is at its warmest temperature.
[3] If siltation continues to worsen, many of the eggs will be smothered, resulting in reduced reproduction, further hindering the eastern sand darters' population count.
[6] The eastern sand darter can be found in many areas throughout the United States and into southern Canada.
In addition to the Mississippi and Ohio Rivers, it can be found in great concentration in Lakes Champlain, Erie, Huron, Michigan and Ontario.
[8] The Eastern sand darter was first formally described as Pleurolepsis pellucidus in 1863 by the American ichthyologist Frederic Ward Putnam (1839–1915) with the type locality given as the Black River at Elyria, Ohio.