Ebenezer Knowlton (December 6, 1815 – September 10, 1874) was a U.S. Representative from Maine, and Free Will Baptist minister.

Born in Pittsfield, New Hampshire, Knowlton moved with his parents to South Montville, Maine, in 1825.

[2] He became an early member of the Republican Party and was a lifelong supporter of abolitionism and the temperance movement.

Knowlton also served as a trustee of Colby College and Maine Central Institute, and after the Civil War he worked for the Freedmen's Bureau in Beaufort, South Carolina.

He was a corporator of the Morning Star, a Free Will Baptist newspaper, and was president of the Foreign Missions Board.