Eduard Knoblauch was born in his family's house on Poststraße 23 in the Nikolaiviertel neighborhood in Berlin, Germany.
He studied at the Berliner Bauakademie under Karl Friedrich Schinkel successfully passing a series of examinations (1818 Feldmesserprüfung, 1822 Kondukteurprüfung, 1828 Baumeisterprüfung).
Together with fellow architect Friedrich August Stüler he traveled in 1829/1830 to France, Switzerland and Italy.
His buildings include: His son Gustav (1833–1916) was also an architect, as was his grandson Arnold (1879–1963), both of Berlin.
His son Carl Eduard (Charles Edward) (1837–1886) was a businessman in London and New York City.