As director of the Buenos Aires Zoological Garden he greatly developed its scientific aspect, publishing booklets and providing printed media for a learned appreciation of its contents.
He also directed the Natural History Cabinet of the University of Buenos Aires and published the standard reference works on botany and zoology used in his country for most of the 20th century.
Coming from a European Bourgeois family, Holmberg had mastered English, French, and German by the time he became Faculty of Medicine of the University of Buenos Aires.
Periódico Zoológico), two of the most important scientific publications of the era, describing species and investigation the effects of agricultural activities on the natural world.
Holmberg appointed a commission to this effect, composed of Florentino Ameghino, Carlos Berg and Lynch Arribálzaga, to which the current layout is due almost in its entirety.
With the idea of forming a diverse source of zoological training, Holmberg acquired European, African and Asian species to complement the rich fauna of the interior of the country.
He also implemented changes in the treatment and feeding of wild animals, improved their visibility to the public, and encouraged the role of scientific dissemination of the zoological garden before the purely recreational.
In 1909, botanist Cristóbal Mariá Hicken (1875-1933),named a genus of flowering plants from southern South America, (belonging to the family Amaranthaceae) in his honour Holmbergia[1][2]