Eleonore Sophie, Princess of Anhalt-Bernburg (née Princess Eleonore Sophie of Schleswig-Holstein-Sonderburg; 14 February 1603 - 5 January 1675) was a member of the Danish royal family and the consort of Christian II, Prince of Anhalt-Bernburg.

Eleonore was born in Sønderborg on 14 February 1603 to John II, Duke of Schleswig-Holstein-Sonderburg, the third son of Christian III of Denmark and Norway and Dorothea of Saxe-Lauenburg, and Agnes Hedwig of Anhalt, the daughter of Joachim Ernest, Prince of Anhalt.

On 28 February 1625 Eleonore married Prince Christian of Anhalt-Bernburg in Ahrensbök.

After her husband's death in 1656, their son Victor Amadeus became Prince of Anhalt-Bernburg.

She was buried in the crypt of the Castle Church of St.