[3] During her career, Longo Borghini has won the Giro d'Italia Women in 2024, as well as two bronze medals in the road race at the Summer Olympic Games, taking third-place finishes in Rio de Janeiro and Tokyo.

[8][9] Longo Borghini turned professional in 2011, joining the Top Girls Fassa Bortolo team.

[8] In 2016, she won a bronze medal at the individual road race at the Olympic Games in Rio de Janeiro.

In 2017, she won Strade Bianche Women and finished 2nd at the Giro Rosa, as well as winning the Italian rider classification for the third time.

[20][21] In July 2021, she competed in the 137-kilometre (85-mile)-long women's Olympic road race in Tokyo, and won the bronze medal.

She also rode in the mixed team relay at the 2021 UCI Road World Championships in Flanders, where she won the bronze medal.

She rode in the mixed team relay at the 2022 UCI Road World Championships in Wollongong, where she won the silver medal.

[30][31] In August, she skipped Tour de France Femmes due to the lingering effects of a training crash.