Elisabetta was born as the daughter of the procurator Paolo Querini of the Stampalia branch of the family and his wife Bianca Ruzzini, from whose family the Doge Carlo Ruzzini emerged.

In addition to 10 houses near the family palace, Elisabetta owned land near Padua, in Polesine and near Verona, as well as a foundry near Mira.

On March 4, Elisabetta was crowned dogaressa with great pomp in the Doge's Palace with a gold corno studded with precious stones, which was forbidden by a law of 1645.

After the death of her husband in 1700 as a widow, Elisabetta oversaw the construction of a huge funerary monument at San Zanipolo, where she was buried next to the two Valier Doges.

She gave legacies to a number of relatives, friends, churches and charitable institutions, including a sum of 10,000 ducats to the Ospedale der Zitelle.