[1] In 1837 Frezzolini made her professional opera debut in the title role of Bellini's Beatrice di Tenda at the Teatro della Pergola in Florence.

In 1839 she portrayed the title role in the house premiere of Saverio Mercadante's Elena da Feltre at the Teatro Comunale di Bologna.

[1] Frezzolini created the role of Giselda in the world premiere of Giuseppe Verdi's I Lombardi alla prima crociata on 11 February 1843 at La Scala in Milan.

[2] She portrayed Camilla in the world premiere of Saverio Mercadante's Orazi e Curiazi at the Teatro di San Carlo in Naples on 10 November 1846.

On the international stage she made appearances at the Mariinsky Theatre in St. Petersburg, the Royal Opera House in London, the Teatro Real in Madrid, the Theater am Kärntnertor in Vienna, the Théâtre-Italien in Paris, and throughout North America.