Eskdalemuir is a civil parish and small village in Dumfries and Galloway, Scotland, with a population of 265.

The area comprises high wet moorlands, chiefly used for sheep grazing and forestry plantation (Eskdalemuir Forest).

In the community is the Samye Ling Tibetan Buddhist monastery, which incorporates a former hunting lodge called Johnstone House.

Eskdalemuir is rich in archaeological remains, including two neolithic stone circles, the Loupin Stanes and the Girdle Stanes, and bank barrow, Castle O'er, a possible ritual centre for the Selgovae, Raeburnfoot, a Roman fort and later dark age fortifications and settlements.

The inland nature of the climate is more manifest during winter months with frosts being common, and the average lows between December and February are below the freezing point.