[2][3] The Yazoo darter inhabits small, clear, mostly spring-fed streams with substrates that include clay, sand, gravel, or silt.

The range includes Benton, Lafayette, Marshall, Tate, Tippah, and Union counties and parts of Holly Springs National Forest.

[3] Stream channelization and urbanization, particularly culverts have led to range restriction and declining populations of the Yazoo darter.

[4] The species is listed as near-threatened by the International Union for Conservation of Nature, sensitive by the United States Forest Service, globally imperiled by The Nature Conservancy, and vulnerable by the Southeastern Fisheries Council and American Fisheries Society.

[4] The Yazoo darter was first formally described in 1994 by Royal Dallas Suttkus and Henry L. Bart with the type locality given as Hurricane Creek, a tributary to Tallahatchie River in the Yazoo River drainage, at Mississippi Highway 7 in Lafayette County, Mississippi.