Nzilambi received his education at the Johns Hopkins School of Medicine in Baltimore, and was later affiliated with the University of Kinshasa.
The study mainly established the presence of HIV in rural areas for a period of time, as well as the social issues that may have led to the epidemic.
[4] In the late 1980s most of his publications focused on HIV being present in groups of people, including pediatric patients and female prostitutes in Zaire.
As a physician in the 1990s, Nzilambi began a walk-in clinic for Zairean prostitutes in order to help and educate them about HIV.
In the late 1990s, it was discovered that Dr. Nzilambi had moved to the United States and was identifying groups of individuals that were not affected by the disease.