Its genus, Eurycea contains 33 species and includes taxa that have either a metamorphic life cycle or larval-form paedomorphosis.

Intraspecific geographic variation in species of Eurycea has been attributed to several factors: temperature, stream order and productivity of the larval habitat.

Hatchlings emerge with external gills and spend around 2 years fully aquatic larvae before transforming into adults.

Searching males have cirri, mental glands, and protruding premaxillary teeth, which are adapted for locating and courting females in terrestrial habitats.

Guarding males lack these characteristics and instead have seasonally enlarged jaw musculature adapted for defending females at aquatic nesting sites.

[10] The Blue Ridge two-lined salamanders have been reported to the southern Appalachian Mountain range (Sever 1920).

The streams that are more suitable for the Blue Ridge two- lined salamander contain submerged substrate to oviposit.

Blue Ridge two-lined salamanders can reach densities of over 100 individuals in a square meter depending on habitat conditions.

It was found in a study that even with the presence black bellied salamander predators (visually or detected by scent), the use of rocks for cover was not utilized by adults (Sever 1920).

Adult Blue Ridge two-lined salamander primary diet consists of terrestrial organisms such as non insect arthropods, worms, and marine invertebrates.