Eustis High has sports teams for male and female students including basketball, baseball, football, swimming, track, volleyball, weightlifting, softball, cross country, and soccer.

[3] The Eustis High School boys' basketball team is a member of the 5A District 13 Standings in the State of Florida.

Eustis would end up losing in the 3rd round of the playoffs to American Heritage who would go on to win the 5A state title a week later.

[6] The Eustis Panthers ended their 2014–15 season in the 3rd round of the playoffs with a 38–49 loss to Cardinal Gibbons at home.

Six days later Cardinal Gibbons beat Gibbs High School (St. Petersburg, FL) in the final four by a score of 73–38.

The Panthers' only out-of-state game was against East Hall High School, Georgia on December 27, 2012.

East Hall is a school similar in size to Eustis with 1,100 students and is located just outside Gainesville, Georgia[9] In 2010 David Lee announced he was returning to coach the Panthers' boys baseball team in their 2011 season.

Lee led the Panthers to their 2000 state championship win shortly before retiring from Eustis.

Lee would leave the Panthers to coach Leesburg High School for five years and then led the Apopka Blue Darters until 2010.

The Eustis Panthers baseball program has claimed two state Championships in recent history, the first being in 2000 and then a second in 2003.