In neutral desorption EESI (ND-EESI), the liquid for the sample aerosol is provided by a flow of nitrogen.

A room temperature (20 °C) nitrogen gas stream is flowed through a narrow opening (i.d.~0.1 mm) to form a sharp jet targeted at a surface.

Ambient ionization techniques are attractive for many samples for their high tolerance to complex mixtures and for fast testing.

EESI has been employed for the rapid characterization of living objects,[10] native proteins,[11] and metabolic biomarkers.

[16] The maturity of fruit was classified with the combination of EESI and principal component analysis,[17] and live samples were tested a short time later.