Originally from Badakhshan province and ethnically Tajik, Koofi was recently a member of the Afghan delegation negotiating peace with the Taliban in Doha Qatar.

Koofi's father was a Member of Parliament (MP) for 25 years but died at the end of the Afghan war (1979–1989), killed by Mujahideen.

[4] Koofi worked with vulnerable groups such as Internally Displaced People (IDP) and marginalized women and children, and served as a child protection officer for UNICEF from 2002 to 2004.

[4] Koofi began her political career in 2001 after the fall of the Taliban, promoting the right to education of girls in her "Back to school" campaign.

In Parliament, she has focused primarily on women's rights, but she also has legislated for the building of roads to connect remote villages to educational and health facilities.

[7] Koofi intended to run for President of Afghanistan in the 2014 Afghan presidential election on a platform of equal rights for women, promoting universal education, and the opposition to political corruption,[8][9] but she said in July 2014 that the election commission moved the registration date to October 2013 and as a result she did not qualify for the minimum age requirement of 40 years of age.

On 14 August 2020, she was shot in the arm by gunmen, who attempted to assassinate her near Kabul, while she was returning from a visit to the northern province of Parwan with her sister Maryam Koofi.

[1][17] The Favored Daughter: One Woman's Fight to Lead Afghanistan into the Future, is an autobiographical memoir written by Fawzia Koofi with the aid of Nadene Ghouri.