He continued his research in the area of the milieu interieur begun in France by Claude Bernard that compared the life of aquatic and land animals.
Bottazzi published Il metodo sperimentale nelle discipline biologiche in 1906 where he examined experimental methods in biology.
He celebrated the role of Italian scientists Leonardo, Galileo, Spallanzani, and Bufalini and pointed to biology being best explained by mechanistic or physico-chemical phenomena.
Bottazzi became a member of the Commissione dell’alimentazione created to solve problems of malnutrition arising from the sanctions imposed on Italy from 1926.
[1] For his studies on the role of sarcoplasm in muscle contraction, and in the regulation of osmotic pressure in marine animals, between 1925 and 1938 he received three Nobel Prize nominations.