By utilizing the SR 407 to SR 405 route, travelers from Central Florida can access the Kennedy Space Center and the city of Titusville.

State Road 407 was built as a two-lane freeway, meaning that although the highway is expressway-standard, it is merely two lanes with only a dotted or solid yellow line in the middle separating northbound and southbound traffic.

Within the SR 528/SR 407/I-95 triangle is a swampland containing the southern unit of St. Johns National Wildlife Refuge, a popular resting area for migrating birds (the northern unit is two miles (3 km) to the north, adjacent to I-95 and SR 50), and just to the west of the triangle is Tosohatchee Wildlife Management Area, which extends from SR 50 southward to just south of SR 520 in nearby Orange County.

[2] The street is named after Space Shuttle Challenger which disintegrated 73 seconds after launch in 1986.

The northern half of the road lost its two-lane freeway qualities when an at-grade intersection was built just south of SR 405, providing a southern exit to Florida's Spaceport Industrial Park and Space Coast Regional Airport.[when?]