The Florida sand darter (Ammocrypta bifascia) is a species of freshwater ray-finned fish, a darter from the subfamily Etheostomatinae, part of the family Percidae, which also contains the perches, ruffes and pikeperches.

It is endemic to Gulf Coast drainages from the Aplalachicola to the Perdido River in Florida and southern Alabama.

[2] It inhabits streams with waters that are clear to tannin-stained where there are shifting sand bottoms and a moderate to fast flow.

[1] Its appearance is identical to the naked sand darter aside from 2 black bands on each dorsal fin.

[2] The Florida sand darter was first formally described in 1975 by James D. Williams with the type locality given as the Choctawhatchee River, 2.4 kilometres (1.5 miles) west of Pittman, Florida.