In 1941, the streetcar line was replaced by bus service, and it is now operated by the New York City Transit Authority.

Along the way, subway transfers can be made at Court Street–Borough Hall (2, ​3​, 4, ​5​, N, R, and ​W), Jay Street–MetroTech (A, ​C​, F, ​​, N, R, and ​W), DeKalb Avenue (B, ​D, ​N, ​Q, ​R, and ​W), and at various points along the IND Fulton Street Line.

[6] This was at the request of a real estate developer who had paid an annual fee of $90,000 to the Transit Authority to operate the service.

[8] On December 1, 2022, the Metropolitan Transportation Authority released a draft redesign of the Brooklyn bus network.

[9][10] As part of the redesign, the B25 would maintain its existing routing, along with the elimination of closely spaced stops, but would end at Franklin Avenue overnight, with the B26 taking over service on Fulton Street west of this point.