[2] The Seminole killifish ranges throughout much of peninsular Florida from the St. Johns and New river drainages south to the Everglades.

[2] The Seminole killifish occurs in open areas of lakes and quiet pools in streams.

The juveniles are usually encountered in schools in the vicinity of vegetation.

[3] Fundulus seminolis was described by Charles Frédéric Girard in 1859 with the type locality given as Palatka in eastern Florida.

[4] The specific name probably alludes to the Seminole people who are indigenous to southern Florida where this species is endemic.