Gainesboro (/ˈɡeɪnzbʌrə/) is an unincorporated community in Frederick County, Virginia.

Gainesboro is located northwest of Winchester off the North Frederick Pike (US 522) on Gainesboro Road (VA 684).

Gainesboro was established in 1798 and originally known as Pugh Town or Pughtown after an early settler, Job Pugh, who surveyed and plotted the original village.

At the national level, Gainesboro is located in Virginia's 10th congressional district, represented by Democrat Jennifer Wexton as of January 3, 2019.

This Frederick County, Virginia state location article is a stub.