Gargalianoi (Greek: Γαργαλιάνοι) is a town and a former municipality in Messenia, Peloponnese, Greece.

Since the 2011 local government reform it is part of the municipality Trifylia, of which it is a municipal unit.

[3] It is situated 4 km (2.5 mi) from the Ionian Sea coast, 18 km (11 mi) north of Pylos, 21 km (13 mi) south of Kyparissia and 43 km (27 mi) west of Kalamata.

The Greek National Road 9 (Patras - Pyrgos - Pylos) passes through the town.

The municipal unit Gargalianoi is subdivided into the following communities: