He described conjugation in Paramoecium and in his taxonomic approach separated chlorophyll containing flagellates from other protists.
He worked briefly at the Marine Biological Laboratory, Woods Hole, and then continued further studies at Columbia University and obtained a Ph.D. in 1897.
He also took an interest in statistics and worked as a consultant in cancer research with the New York State Department of Health from 1902 to 1908.
One of his most important works was in the study of protistan life-histories and the description of conjugation.
[2] Calkins was elected to the United States National Academy of Sciences in 1919 and the American Philosophical Society in 1920.