It runs from Klamath Falls, Oregon in the north to Keddie, California at the south end.
Historically, the Great Northern laid track from Klamath Falls, Oregon to Bieber, California and the Western Pacific laid track from the famed Keddie Wye near Keddie, California to Bieber (Actual town is known as Nubieber, today.
[3] Traffic on the Gateway Subdivision consists of general merchandise freights and empty well car trains—as many as three of the latter each day.
As of October 2011[update], there is one local freight that runs out of Klamath Falls twice weekly—south to Clear Creek Jct.
Amtrak's Seattle–Los Angeles Coast Starlight stops at Klamath Falls, but there is no passenger service on the Gateway Subdivision itself.