[3] Bartley is also the Louis and Evelyn Krueger Professor of Ophthalmology at the Mayo Clinic in Rochester, Minnesota.
After internship at Riverside Methodist Hospital in Columbus, Ohio, he completed residency training in ophthalmology at the Mayo Clinic and subspecialty training in ophthalmic plastic and orbital surgery at Wright State University under John D. Bullock, a cataract surgeon and ophthalmic plastic surgeon.
The following year, Bartley was appointed chief executive officer of Mayo's operations in Florida.
He resumed his surgical practice in Rochester in 2009 while serving as Chair of the Doctors Mayo Society and Medical Director for Alumni Philanthropy.
[citation needed] Bartley has served as the editor-in-chief of Ophthalmic Plastic and Reconstructive Surgery, Senior Associate Editor of the American Journal of Ophthalmology, and as an editorial board member of Archives of Ophthalmology.