He matriculated at St Edmund Hall, Oxford on 24 March 1721, aged 17.
[2] Chamberlayne was returned as a Whig Member of Parliament for Buckingham at a by-election on 20 February 1728 on the interest of his uncle, Alexander Denton.
In Parliament he voted with the Administration on the army 1732[clarification needed] and on the Excise Bill in 1733.
He was returned unopposed for Buckingham at the 1734 British general election and went into opposition, probably under Lord Cobham and the Grenvilles.[who?]
He died on 14 May 1757 leaving one daughter, Elizabeth, who married Wenman Coke.