In Gwinnett County, SR 365 begins concurrent with I-985 at an interchange with I-85 on the southeastern edge of Suwanee.

Nearly 2.5 miles (4.0 km) later US 129 departs the concurrency to the north with SR 369 on Jesse Jewell Parkway.

East of there, it passes a Kubota RTV manufacturing plant, and a cold storage warehouse.

A much more important intersection is encountered later on when the routes pass by Ace Hardware franchise, and a gift shop and furniture store just before approaching SR 52 (Lula Road) northwest of Lula, which leads to Clermont.

Beyond this point it continues to run up and down the hills of Hall County passing through more minor intersections.

[1] A little ways later, the highways enter Habersham County where the road is named the Tommy Irwin Parkway.

Just before entering the northern part of Cornelia, passing a bridge over J. Warren Road with no access.

Right before entering the Chattahoochee-Oconee National Forest, US 23/US 441/SR 15 heads north, while SR 365 continues to the northeast, along with US 123.

The three highways curve to the southeast into Stephens County until they meet SR 184 (Dicks Hill Parkway).

Farther to the northeast, in Toccoa, they intersect SR 63/SR 106 (South Broad Street).

After leaving Toccoa, the two highways continue to the northeast until they meet SR 365's northern terminus, the South Carolina state line at the Tugaloo River, southwest of Westminster, South Carolina, where US 123 continues toward the town.

[1] Except for the extreme northern section, all of SR 365 is included as part of the National Highway System, a system of roadways important to the nation's economy, defense, and mobility.

In addition, the entire route, from Buford to the South Carolina state line, was paved.

[10][11] In 1935, after a long series of improvement projects the section just south of Buford was paved.

[20][21] In 1966, SR 365 was being projected as a freeway from its current southern terminus northeast and curving around the southeastern side of Gainesville.