Gertrude Anne of Hohenberg (c. 1225 – 16 February 1281) was German queen from 1273 until her death, by her marriage with King Rudolf I of Germany.

The comital Hohenberg dynasty, a cadet branch of the Swabian House of Hohenzollern, then ruled over extended estates in southwestern Germany.

Citing contemporary sources, Gertrude's descent was questioned by the Swiss historian Aegidius Tschudi (1505–1572), who postulated a Frohburg lineage; nevertheless, his objections have been disproved.

About 1253/4, Gertrude married Rudolf (1218–1291),[2] son of Count Albert IV of Habsburg and Heilwig of Kyburg.

Reluctant to interfere in politics, she witnessed Rudolf's struggles to secure his rule against the rivalling King Ottokar II of Bohemia, as well as his fruitless efforts to be crowned Holy Roman Emperor.