He was the eldest son of Leonard Poole (d. 1538) of Sapperton by Catherine, the daughter of Sir Giles Brydges of Coberley.
[1] He married Elizabeth (d. 1543), one of the 6 daughters and co-heiresses of Thomas Whittington of Pauntley with whom he had a son Henry.
[1] He sat for Gloucestershire in Mary I's second parliament and was appointed provost marshal for Ireland in 1558, suggesting he supported the restoration of Catholicism.
He married Eleanor, daughter of Edward Lewknor of Kingston by Sea, Sussex and widow of Sir William Wroughton (d. 1559) of Broad Hinton, Wilts.
[2] His will[3] refers to the funeral monument he had erected at Sapperton, making it likely that he is represented by the effigy on the tomb dated 1574 there.