The organization's mission was to help young women who had migrated from rural communities in search of job opportunities, experiencing upheaval in the aftermath of the Civil War.

The National Council, composed of 300 voting members, makes decisions concerning the purpose, goals, and public policies of the organization.

"[10] In September 2006, Warren Buffett auctioned his Lincoln Town Car to support Girls Inc.

[15] Girls Inc. often participate in efforts with partner companies that promote positive change for women, often through events such as raising money through charity sales or donating.

Some of Girls Inc.’s partner companies include Dove, Cummins,[16] Freeform, Business Wire, Motorola Foundation, American Chemical Society, NPower, Adventures of the Mind, Nonprofit VOTE and Space Science Laboratory.

[17] A venture with the American Girl Dolls collection in 2005 generated controversy among fundamentalist Christians.

"[18] In 1970s, the organization developed a number of programs in six main areas: 1) careers and life planning, 2) health and sexuality, 3) leadership and community action, 4) sports and adventure, 5) self-reliance and life skills, and 6) culture and heritage.