He also painted frescoes on a vaulted room of the residence of the Puricelli Guerra, representing the great men of the Middle Ages, backgrounded against perspectives of Gothic architecture.

He painted a Torquato Tasso introduced to Emmanuel Philibert; Death of St Joseph (commissioned by a parish in Palermo; an Assumption of the Virgin for a church in Valmarana in Altavilla Vicentina; an altarpiece of Vision of St Francis of Assisi for the church of San Babila, Milan; paintings for the palazzo of Count Ernesto Turati in Milan; and frescoes for the house of Cavalier Andrea Ponti in Varese representing Guido d'Arezzo teaching singing to a children's choir, as well as scenes from the life of Volta, Galileo, and Columbus.

The Sala Dorata in the Poldi-Pezzoli Museum at Milan has a series of three mural panels by Bertini at the end of the room opposite the window, the central one representing Painting, Sculpture, and Architecture, and the two lateral ones symbolizing Poetry and Music.

Giuseppe Barbaglia, Cesare Bertolotti, Emilio Cavenaghi, Francesco Filippini, Andrea Fossati,[3] Pietro Michis, and Lodovico Pogliaghi were among his pupils.

Pompeo Bertini, his brother, made stained glass windows, sometimes using designs by Giuseppe.