Glenroy station is currently served by the Craigieburn line, part of the Melbourne railway network.

The station is approximately 15 km (9.3 mi) or around a 32 minute train ride to Flinders Street via the City Loop.

[4] The station is 15 km (9.3 mi) or around a 32 minute train ride to Flinders Street via the City Loop.

[11] Like the suburb itself, the station is named after a pastoral run occupied by Duncan Cameron, who originated from Glen Roy, Scotland.

Hand gates protected the former Glenroy Road level crossing until 1957, when boom barriers were provided.

[18] On 2 July 2019, it was announced that the level crossing would be removed by lowering the railway line underneath Glenroy Road, and would include a rebuilt station.

[7] The Craigieburn line runs from Craigieburn station, north of Melbourne, joining with the Upfield, Sunbury, Werribee and Williamstown lines at North Melbourne station before travelling through to Flinders Street via the City Loop in a clockwise direction during weekday morning peak hours or all day on weekends and public holidays and to Flinders Street via Southern Cross in an anti-clockwise direction during weekday afternoon and evening peak hours.