Johnson was the Democratic Party nominee for the 2024 United States Senate election in Tennessee, losing to incumbent Republican Marsha Blackburn.

Johnson instead moved her desk to the hallway outside and gave the room to her assistant, who was originally given a non-connecting closet for her office.

[12][13] During her interview with Mother Jones a day before her expulsion vote, Johnson accused House Republicans of fascism and said she believed Tennessee was less democratic than North Korea.

[16] In late July 2023, it was reported Johnson would launch a campaign the following month to challenge Senator Marsha Blackburn in the 2024 election.

[19] In March 2024, Grammy-winning vocalists Brandi Carlile, Emmylou Harris, Maren Morris, and Allison Russell, along with more than 30 other artists, came together to record and release a new "soul anthem" called Tennessee Rise in support of Johnson's senate campaign.

[21] She defeated previous Democratic Nominee for U.S. Senate in 2020, Marquita Bradshaw, along with Civil Miller-Watkins and Lola Denise Brown.