[2] Gray four-eyed opossums do not have a well defined territory, and home range stability depends on the availability of adequate resources.

[4] They are omnivorous, feeding on small animals and vegetation, such as leaves, seeds, and fruits.

[6] Little is known about gray four-eyed opossums mating habits but they are in the family Didelphidae and so most likely to be polygynous.

[5] During the dry season, fewer babies are born due to the lack of available fruit.

[6] The average gestation period for the gray four-eyed opossum is 13 to 14 days, and each newborn weighs about 9 grams (0.32 oz).

[5] The gray four-eyed opossum has an omnivorous diet containing fruits, nectar, insects, small mammals (such as mice), birds, reptiles, amphibians, crustaceans, snails, and earthworms.

[6] With such a varied diet, the gray four-eyed opossum will both encounter and eat venomous snakes.

While the bites of these snakes may be harmful to most animals, the gray four-eyed opossum is able to overcome the toxic effects due to its immunity to the toxins.

[7][8] These proteins are produced by the opossum prior to any encounter with a venomous snake, thus this immunity is not learned but inherited.