[1] The titular squad consists of three high school cheerleaders and best friends who gain superpowers after accidentally being zapped by a jolt of electricity from the mad scientist Dr. Nightingale.

With help from their team mascot and their hairstylist, a former Mi6-agent, they must learn to use their newfound powers to defeat Dr. Nightingale while hiding their identities as superheroes and win the annual cheerleading competition.

With help from Adrienne and Zeke, the team mascot for Bay City High School, they fight against Dr. Nightingale, who is the father of their fellow cheerleader Star, to thwart his plans for world domination.

She is the captain of Bay City High School's cheerleading team and the leader of the Groove Squad, and possesses the power of superhuman strength.

Zeke (voiced by Andrew Francis): The closest male friend of the Groove Squad, who is the team mascot of Bay City High School.

Adrienne (voiced by Blu Mankuma): A hairstylist from England who provides the Groove Squad with special containers that hold their fruit smoothies and allow them to transform into superheroes.

Flower Power: A trio of superpowered hippies who Dr. Nightingale hires to distract the Groove Squad and prevent them from interfering in his plans and to help Star become head cheerleader at Bay City High School.

Stacy and Roxanne (voiced by Vanessa Morley and Kathleen Barr, respectively): Identical twin sisters who are Star's best friends and Chrissy, Ping and Mackenzie's fellow cheerleaders.

Before the Groove Squad manages to return in time for the competition and Star declares herself head cheerleader, they are unsure about having her as their new team captain, but eventually accept her.

Fernando (voiced by Santo Lombardo): A Spanish-speaking exchange student and player on the Bay City High School football team.