The Gualino Madonna is a painting attributed to Italian late medieval artist Duccio di Buoninsegna.

[1] It is housed in the Galleria Sabauda of Turin, northern Italy.

In 1910 it was sold on the Florence antiquary market covered by a 16th-century repainting, which was removed in 1920.

In 1925 it was acquired by the Turinese entrepreneur and collector Riccardo Gualino who, in 1930, gave it to the Galleria Sabauda.

Now it is unanimously assigned to Duccio, belonging to his early career, when he was influenced by Cimabue.