Guatemala participated at the 2018 Summer Youth Olympics in Buenos Aires, Argentina from 6 to 18 October 2018.

Guatemala qualified a girls' team based on their performance at the 2018 AFECAVOL Zone U19 Championship.

[1] Guatemala qualified one gymnast based on its performance at the 2018 American Junior Championship.

[2] Guatemala qualified one athlete based on its performance at one of the Karate Qualification Tournaments.

[3] Guatemala qualified one boat based on its performance at the American Qualification Regatta.