Guglielmo Gasparrini (3 January 1803 – 28 June 1866) was an Italian botanist and mycologist.

After his first studies in his native town, he moved to Naples to attend the veterinary school.

Subsequently, he entered the Botanical Garden of Naples, under the guide of Michele Tenore and Giovanni Gussone.

Graduating in the physical and natural sciences, he was professor of botany at the University of Naples.

From 1857 to 1861, he taught at the University of Pavia, of which he was also magnificent rector (Magnífico Reitor).