He later started a public course in his laboratory in 1738, in 1742 he was appointed experimental demonstrator of chemistry at the Jardin du Roi in Paris.

he was especially influential and popular as a teacher, and taught many students among whom were Denis Diderot, Antoine-Laurent de Lavoisier, Joseph Proust and Antoine-Augustin Parmentier.

In addition to his investigation of neutral salts, he published papers on the inflammation of turpentine and other essential oils by nitric acid, and the methods of embalming practised in Ancient Egypt.

[3] In this paper, which was an extension of an earlier memoir on the same subject written in 1744, Rouelle pointed out that the number of known salts had increased significantly during the 17th and early 18th centuries.

This was due not only to the preparation of new salts, but also to an increasing ability to distinguish between sodium and potassium compounds, and to a generalization of the concept so as to include many substances, such as the alums and vitriols (i.e., sulfates), that had been previously excluded.