It was built by the Koninklijke Maatschappij De Schelde of Vlissingen[1] and entered active duty on 4 March 1934.

[2] On 22 October 1940, O 14 arrived in Rothesay, Bute, and with O 9 and O 10 was used as a target ship during tests with the ASDIC sonar system.

In January 1942 O 14 participated in Operation Kitbag, where it was placed 15 nautical miles (28 km; 17 mi) off the Norwegian coast and used as a radio beacon for destroyers on their way to the fjord at Hell, Norway.

At the end of January and the start of February O 14 was involved with the unsuccessful search for the German battleship Tirpitz.

The Dutch crew was then stationed on a sub loaned from the British Navy, HMS Sturgeon, which was renamed Zeehond.