[2] His brother Raimond d'Estienne became the heir of the Estienne family, while Henri was disinherited by his father in 1482[2] "for having devoted himself to printing", the profession of printer then being the cause of losing your title.
[10] Estienne's printmark was of the old arms of the University, a shield charged with three Fleurs de Lis, with a hand emerging from a cloud and holding a closed book[11] with the motto was: Plus olei quam vini ("more oil than wine,").
[16] Along with de Colines, Estienne helped to move the printing type style from gothic to roman.
[17] Philippe Renouard attributed the Estienne print shop with publishing over 1590 editions (an average of nine per year) from 1502 to 1664.
[15] Among his publications were the editions of Aristotle,[19] and the Psalterium quincuplex, of Lefèvre d'Estaples, 1509 & 1513,[20] and his commentary on the Pauline Epistles in 1512.
[23] Estienne also published an influential print of Materia medica, translated by Jean Ruel, in 1516.
[13] His widow Guyonne Viart married Simon de Colines, a remarkable printer of the 16th century.
[16] Charles Estienne was a medical scholar, even publishing some works related to medicine and natural history.