High Five (BTI-Tn-5B1-4) is an insect cell line that originated from the eggs of the cabbage looper, Trichoplusia ni.
[1] It was developed by the Boyce Thompson Institute for Plant Research.
[2][3][4][5][6][7][8] The High Five cells have been used to produce the VLP-based HPV vaccine Cervarix.
[9] They can be grown in the absence of serum, and can be cultured in a loose attached state or in suspension[10] High Five cells produce abundant microRNAs (miRNAs), small interfering RNAs (siRNAs), and PIWI-interacting RNAs (piRNAs), making it suitable to study all three types of small silencing RNAs.
Researchers at the Boyce Thompson Institute were able to isolate virus-free sub-clones, named Tnao38[13][14] and Tnms42.