Services that formerly terminated at Malin Bridge and Forbes Road were diverted into the Interchange, and services that formerly stopped further up the road by Hillsborough Barracks near the old Officer Mess building (now the headquarters for Sheffield Insulations Ltd) were also re-routed to stop at the Interchange.

The platform which serves trams heading towards Malin Bridge and Middlewood is located in front of The Rawson Spring public house, with the platform serving trams towards Halfway and Meadowhall Interchange being located opposite.

Stands H4 and H5 have the same continuous shelter and run at 45 degrees from the travel centre to the cycle parking at rear of the bus station.

As of January 2021[update], the stand allocation is: South Yorkshire Supertram operate two routes through Hillsborough Interchange.

The Blue route continues north to Malin Bridge and south to Halfway via the city centre, whilst the Yellow route runs between Middlewood in the west and Meadowhall Interchange in the east, again via the city centre.