His most celebrated work, Jeune Homme Nu Assis au Bord de la Mer (1836) is held in the Louvre.

Hippolyte and Paul spent some time at Lyon, saving to leave for Paris in 1829 and study under Louis Hersent.

Dante and Virgil visiting the Envious Men struck with Blindness and Euripides writing his Tragedies are now in the Museum of Fine Arts in Lyon.

Their son Paul Hippolyte Flandrin (1856–1921) became a painter of sacred art, portraitist and decorator.

In 1863, Flandrin's failing health, made worse by his hard work and extended exposure to the damp and draughts of churches, induced him to visit Italy again, where he died of smallpox in Rome on 21 March 1864.