Holmes Valley is an unincorporated community in Washington County, Florida on State Hwy 279.

It was founded in 1848 after federal troops pursued Creek Indian Chief Holmes there and killed him on the spot.

Robert Edwin Russ, the founder of Ruston, Louisiana, was born in Holmes Valley in 1830 but soon moved to Tennessee, Alabama, Mississippi, and Louisiana.

Hand-planed tongue-and-groove planks were laid down, and wooden nails and square pegs were used in its construction.

This article about a location in Washington County, Florida is a stub.